Reflection: Classroom Setup Purification of mFP from an Overnight Culture - Section 1: Introduction


In this lab you and your students have an opportunity to handle a known biohazard in a safe manner using the ascetic technique that has been in the backdrop during the previous labs. Because you are working with transformed bacteria that may possess multiple antibiotic resistances it is imperative that everyone has the proper PPE (Personal Protective Equipment) which includes approved goggles, gloves, and an apron or lab coat.

Review with students any liquid or material that has come in contact with the bacterial cells will need to be handled in a special way. Items such as tips must be disinfected using 10% bleach and placed in a biohazard bag for pickup. 

Students will also be using a high speed table top centrifuge which seems harmless however a search on YouTube may provide some inspiration for NOT taking this piece of equipment for granted. It is a great idea to review the proper way to balance and load a centrifuge BEFORE closing the lid which may LOCK on some models of this lab tool!!

  Nobel Prize Lab Teacher's Notes - Volume 6
  Classroom Setup: Nobel Prize Lab Teacher's Notes - Volume 6
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Purification of mFP from an Overnight Culture

Unit 8: Nobel Prize-Winning Biotechnology
Lesson 6 of 9

Objective: Students will be able test a method or technique for purifying (separating) a protein of interest from other gene products in a simulated cell/protein sample.

Big Idea: Differences can be grand! In order to purify an individual protein present in a cell we must know how use the differences in proteins as the selection factor.

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