Reflection: Creating a Cell Culture of Escherichia coli - Section 1: Introduction


After completing the preparation for Lab 5 and then conducting the bacterial transformation it is great to get to this lab which has very little prep. The amount of sterile LB/amp/ara broth you prepare should be based on the number of student groups you have in each class period. A good estimate is to prepare 3.0mL per group. 

The overnight culture is really a cool change to witness so be sure to take a before and after photo to discuss with your students as many will forget what the culture looked like a day or two before. Finally, the bacteria in your flask are living organisms and they need to eat, breath and multiply. Be mindful NOT to put the cap on too tight to that the inhabitants can't breath yet not too loose so that it falls off due being placed overnight on the shaker. It should be snug BUT not tight!

  Nobel Prize Lab Teacher's Notes - Volume 5
  Nobel Prize Lab Teacher's Notes - Volume 5
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Creating a Cell Culture of Escherichia coli

Unit 8: Nobel Prize-Winning Biotechnology
Lesson 5 of 9

Objective: Students will be able to use the technique of cell culturing to produce a greater number of bacterial cells which carry the gene for a mutant florescent protein.

Big Idea: In cell culturing genes don't "lie" they "multiply"... and create PROTEIN!

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Science, Biotechnology, DNA Technology
  135 minutes
overnight cell
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