Reflection: Classroom Setup pARA-R Restriction Digest: An Introduction to Plasmids and Restriction Enzymes - Section 1: Introduction



Most of the instructors time in preparation of this lab will involve aliquoting solutions. A time saving tip is to have a few students label the tubes BEFORE aliquoting the plasmids and buffers into the tubes and to practice aliquoting on ice as a best practice.

As students are working through this protocol keep a watchful eye on their micropipetting technique. The reagents for these labs are expensive and so aspirating more solution than needed may result in running short on reagents at a time when you least expect be certain that students are pipetting correctly.

This is a great time to remind students of labeling as well! Be certain that students are labeling their tubes with the group number AND class period so that they are easily located at then end of a reaction or the next lab day. Finally, also remind students that incubation times are not advisory in nature and should be adhered to at all "times". In this particular lab remember to remove the students restriction digests from the 37C water bath after one hour. If the samples remain in the hot water bath for more than one hour it is possible that one of the restriction enzymes will begin cutting the DNA randomly and not just at the normal restriction sites. As a reminder, I set a timer for one hour and place the class period on a piece of tape that I affix to the front of the timer. Mostly likely I will need to remove a previous class periods samples during a later class period due to the extended incubation time and this helps to keep everything straight. 

  Nobel Prize Lab Teachers Notes - Volume 1
  Classroom Setup: Nobel Prize Lab Teachers Notes - Volume 1
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pARA-R Restriction Digest: An Introduction to Plasmids and Restriction Enzymes

Unit 8: Nobel Prize-Winning Biotechnology
Lesson 1 of 9

Objective: Students will be able to examine the role of restriction enzymes (RE's) and their importance in genetic engineering as well as demonstrate their understanding by using the RE's BamHI and HindIII to isolate a gene!

Big Idea: Discover the two most powerful yet fundamental tools used in biotechnology!

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Science, Biotechnology, DNA Technology
  145 minutes
proper pipetting
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